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the scrambled control shrna vector (shcontrol)  (Genechem)

 
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    Structured Review

    Genechem the scrambled control shrna vector (shcontrol)
    Cell proliferation and apoptosis assay results.
    The Scrambled Control Shrna Vector (Shcontrol), supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/the+scrambled+control+shrna+vector+%28shcontrol%29/pmc05779981-49-13-20?v=Genechem
    Average 90 stars, based on 1 article reviews
    the scrambled control shrna vector (shcontrol) - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "HMGA1 participates in MHCC97H cell proliferation and invasion through the ILK/Akt/GSK3β signaling pathway"

    Article Title: HMGA1 participates in MHCC97H cell proliferation and invasion through the ILK/Akt/GSK3β signaling pathway

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2017.7820

    Cell proliferation and apoptosis assay results.
    Figure Legend Snippet: Cell proliferation and apoptosis assay results.

    Techniques Used: Apoptosis Assay

    The effects on apoptosis in MHCC97H hepatocellular carcinoma cells treated with shHMGA1, ILK expression vector and/or MK2206. In each panel, B2 quadrant indicates only PI positive cells that were necrotic and the B4 quadrant indicates live cells. The B1 quadrant indicates Annexin and PI as late apoptosis cells and the B3 quadrant illustrates Annexin as early apoptotic cells. Apoptosis levels were also detected by flow cytometry in each of the seven experimental groups: (A) Blank control group; (B) shControl group; (C) shHMGA1 group; (D) shHMGA1 + EGFP group; (E) shHMGA1 + ILK group; (F) shHMGA1 + ILK + DMSO group; and (G) shHMGA1 + ILK + MK2206 group. DMSO, dimethylsulfoxide; EGFP, enhanced green fluorescent protein; HMGA1, high mobility group AT-hook 1; ILK, integrin-linked kinase; MK2206, an Akt-specific inhibitor; sh, short hairpin RNA.
    Figure Legend Snippet: The effects on apoptosis in MHCC97H hepatocellular carcinoma cells treated with shHMGA1, ILK expression vector and/or MK2206. In each panel, B2 quadrant indicates only PI positive cells that were necrotic and the B4 quadrant indicates live cells. The B1 quadrant indicates Annexin and PI as late apoptosis cells and the B3 quadrant illustrates Annexin as early apoptotic cells. Apoptosis levels were also detected by flow cytometry in each of the seven experimental groups: (A) Blank control group; (B) shControl group; (C) shHMGA1 group; (D) shHMGA1 + EGFP group; (E) shHMGA1 + ILK group; (F) shHMGA1 + ILK + DMSO group; and (G) shHMGA1 + ILK + MK2206 group. DMSO, dimethylsulfoxide; EGFP, enhanced green fluorescent protein; HMGA1, high mobility group AT-hook 1; ILK, integrin-linked kinase; MK2206, an Akt-specific inhibitor; sh, short hairpin RNA.

    Techniques Used: Expressing, Plasmid Preparation, Flow Cytometry, shRNA



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    Image Search Results


    Cell proliferation and apoptosis assay results.

    Journal: Molecular Medicine Reports

    Article Title: HMGA1 participates in MHCC97H cell proliferation and invasion through the ILK/Akt/GSK3β signaling pathway

    doi: 10.3892/mmr.2017.7820

    Figure Lengend Snippet: Cell proliferation and apoptosis assay results.

    Article Snippet: The lentiviral short hairpin (sh)RNA vector against HMGA1 (shHMGA1, 5′-GATCCAGCGAAGTGCCAACACCTATTCAAG-AGATAGGTGTTGGCACTTCGCTTTTTTTA-3′) and the scrambled control shRNA vector (shControl) were obtained from GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Apoptosis Assay

    The effects on apoptosis in MHCC97H hepatocellular carcinoma cells treated with shHMGA1, ILK expression vector and/or MK2206. In each panel, B2 quadrant indicates only PI positive cells that were necrotic and the B4 quadrant indicates live cells. The B1 quadrant indicates Annexin and PI as late apoptosis cells and the B3 quadrant illustrates Annexin as early apoptotic cells. Apoptosis levels were also detected by flow cytometry in each of the seven experimental groups: (A) Blank control group; (B) shControl group; (C) shHMGA1 group; (D) shHMGA1 + EGFP group; (E) shHMGA1 + ILK group; (F) shHMGA1 + ILK + DMSO group; and (G) shHMGA1 + ILK + MK2206 group. DMSO, dimethylsulfoxide; EGFP, enhanced green fluorescent protein; HMGA1, high mobility group AT-hook 1; ILK, integrin-linked kinase; MK2206, an Akt-specific inhibitor; sh, short hairpin RNA.

    Journal: Molecular Medicine Reports

    Article Title: HMGA1 participates in MHCC97H cell proliferation and invasion through the ILK/Akt/GSK3β signaling pathway

    doi: 10.3892/mmr.2017.7820

    Figure Lengend Snippet: The effects on apoptosis in MHCC97H hepatocellular carcinoma cells treated with shHMGA1, ILK expression vector and/or MK2206. In each panel, B2 quadrant indicates only PI positive cells that were necrotic and the B4 quadrant indicates live cells. The B1 quadrant indicates Annexin and PI as late apoptosis cells and the B3 quadrant illustrates Annexin as early apoptotic cells. Apoptosis levels were also detected by flow cytometry in each of the seven experimental groups: (A) Blank control group; (B) shControl group; (C) shHMGA1 group; (D) shHMGA1 + EGFP group; (E) shHMGA1 + ILK group; (F) shHMGA1 + ILK + DMSO group; and (G) shHMGA1 + ILK + MK2206 group. DMSO, dimethylsulfoxide; EGFP, enhanced green fluorescent protein; HMGA1, high mobility group AT-hook 1; ILK, integrin-linked kinase; MK2206, an Akt-specific inhibitor; sh, short hairpin RNA.

    Article Snippet: The lentiviral short hairpin (sh)RNA vector against HMGA1 (shHMGA1, 5′-GATCCAGCGAAGTGCCAACACCTATTCAAG-AGATAGGTGTTGGCACTTCGCTTTTTTTA-3′) and the scrambled control shRNA vector (shControl) were obtained from GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Expressing, Plasmid Preparation, Flow Cytometry, shRNA